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Human MIP5 ELISA Kit (CCL15) is a Sandwich (quantitative) ELISA for the measurement of Human MIP5 (CCL15) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
MIP5, NCC3, SCYA15, CCL15, C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, Small-inducible cytokine A15, HCC-2, LKN-1, MIP-5
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 3 pg/mL
4.1 - 1000 pg/mL
Microplate
Abcam's MIP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP5 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human MIP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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Representative Standard Curve using ab100598.
Representative Standard Curve using ab100598.
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