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Human TIMP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human TIMP1 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CLGI, TIMP, TIMP1, Metalloproteinase inhibitor 1, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, Tissue inhibitor of metalloproteinases 1, EPA, Collagenase inhibitor, TIMP-1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 40 pg/mL
24.69 - 18000 pg/mL
Microplate
Abcam's TIMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human TIMP1 ELISA Kit (ab187394) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
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-20°C
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Representative standard curve using ab100651
Representative standard curve using ab100651
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