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Human PLGF ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PLGF in Human in Biofluids, Cell Culture Media samples.
隐藏别名
PGFL, PLGF, PGF, Placenta growth factor, PlGF
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 2 pg/mL
1.372 - 1000 pg/mL
Microplate
Abcam's PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100629.
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