| 存储条件 | -20℃ |
|---|
Human LYVE ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human LYVE in Human in Biofluids, Cell Culture Media samples.
隐藏别名
CRSBP1, HAR, XLKD1, UNQ230/PRO263, LYVE1, Lymphatic vessel endothelial hyaluronic acid receptor 1, LYVE-1, Cell surface retention sequence-binding protein 1, Extracellular link domain-containing protein 1, Hyaluronic acid receptor, CRSBP-1
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 8 pg/mL
8.19 - 2000 pg/mL
Microplate
Abcam's Human LYVE ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LYVE in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human LYVE coated on a 96-well plate. Standards and samples are pipetted into the wells and LYVE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LYVE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LYVE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative Standard Curve using ab100585.
Representative Standard Curve using ab100585.
ab100585 was used to detect LYVE in Human heparin plasma samples according to the kit protocol. 10 μL sample was used per well.
抱歉，暂无相关文献
