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Human IL-4 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human IL-4 in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 5 pg/mL
3.13 - 200 pg/mL
Microplate
Abcam's IL-4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-4 coated on a 96-well plate. Standards and samples are pipette into the wells and IL-4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
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-20°C
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Representative standard curve using ab100570
Representative standard curve using ab100570
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