| 存储条件 |
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| Product Name | PELP1 [D11-D6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | glutamic acid- and leucine-rich protein 1 antibody HMX3 antibody MNAR antibody Modulator of non-genomic activity of estrogen receptor antibody Modulator of nongenomic activity of estrogen receptor antibody P160 antibody Pelp1 antibody PELP1 proline glutamic acid leucine rich protein 1 antibody PELP1_HUMAN antibody proline and glutamic acid rich nuclear protein antibody Proline glutamate and leucine rich protein 1 antibody Proline- antibody proline-, glutamic acid- and leucine-rich protein 1 antibody Transcription factor HMX3 antibody |
| Product description | The estrogen receptor (ER) plays an important role in cancer progression. PELP1/MNAR (proline-, glutamic acid- and leucine-rich protein-1/modulator of non-genomic activity of estrogen receptor), a novel coregulatory protein, modulates genomic as well as nongenomic activity of estrogen receptors. PELP1 plays an essential role in the proliferation of cancerous endometrial cells. PELP1 expression, in both the stroma and epithelial cells, and localization are widely deregulated in endometrial cancers. In addition, PELP1 and ER beta localize predominantly in the cytoplasm of high-grade endometrial tumors. PELP1 coactivates ER-mediated transcription and also serves as a corepressor of other nuclear hormone receptors (NR)- and non-NR sequence-specific transcription factors, including GR, Nur77, AP1, NFkappaB, and TCF/SRF. PELP1 participates in chromatin remodeling activity via displacement of histone 1 in cancer cells and is expressed in all stages of endometrium. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | WBFC |
| WB:1:500-1:2,000 FC:1:100-1:200 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | glutamic acid- and leucine-rich protein 1 antibody HMX3 antibody MNAR antibody Modulator of non-genomic activity of estrogen receptor antibody Modulator of nongenomic activity of estrogen receptor antibody P160 antibody Pelp1 antibody PELP1 proline glutamic acid leucine rich protein 1 antibody PELP1_HUMAN antibody proline and glutamic acid rich nuclear protein antibody Proline glutamate and leucine rich protein 1 antibody Proline- antibody proline- glutamic acid- and leucine-rich protein 1 antibody Transcription factor HMX3 antibody |
|---|---|
| Molecular Weight(MW) | 120 kDa |
| Cellular Localization | Nucleus. Cytoplasm.? |
| SwissProt ID | Q8IZL8 |
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Application
Fig1: Western blot analysis of PELP1 on human PELP1 recombinant protein using anti-PELP1 antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of PELP1 on HEK293 (1) and PELP1-hIgGFc transfected HEK293 (2) cell lysate using anti-PELP1 antibody at 1/1,000 dilution.
Application
Fig3:Flow cytometric analysis of Hela cells with PELP1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human PELP1 recombinant protein, PELP1-hIgGFc transfected HEK293 cell lysate,Hela. |
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| Application Notes | WB:1:500-1:2,000 FC:1:100-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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