| 存储条件 |
|---|
| Product Name | VIMP [A10-G4] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AD-015 antibody ADO15 antibody MGC104346 antibody MGC2553 antibody SBBI8 antibody selenoprotein S antibody SEPS1 antibody VCP interacting membrane protein antibody VCP-interacting membrane antibody VIMP antibody |
| Product description | Selenium is an essential trace element that is incorporated as selenocysteine into the primary structure of Selenoproteins. Nutritional deficiency of selenium decreases Selenoprotein concentrations and leads to pathologic conditions. Most of the known Selenoproteins are members of the glutathione peroxidase or iodothyronine deiodinase families. Selenoprotein S, also known as VIMP or SELS, is a 189 amino acid single-pass membrane protein that localizes to the endoplasmic reticulum (ER) and contains a selenocysteine (Sec) residue at its active site. Interacting with Derlin-1 and VCP, Selenoprotein S is involved in the degradation of misfolded ER proteins, specifically participating in the transfer of misfolded proteins from the ER to the cytosol for subsequent proteasomal degradation. Aberrant expression of Selenoprotein S is associated with diabetes, cardiovascular disease and rheumatoid arthritis. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | Human |
| Concentration | 2mg/ml |
| Alternative Names | AD-015 antibody ADO15 antibody MGC104346 antibody MGC2553 antibody SBBI8 antibody selenoprotein S antibody SEPS1 antibody VCP interacting membrane protein antibody VCP-interacting membrane antibody VIMP antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa |
| Cellular Localization | Endoplasmic reticulum membrane. Cytoplasm. |
| SwissProt ID | Q9BQE4 |
|---|
Application
Fig1: Western blot analysis of VIMP on human VIMP recombinant protein using anti-VIMP antibody at 1/1,000 dilution.
Application
Fig2: Western blot analysis of VIMP on HEK293 (1) and VIMP-hIgGFc transfected HEK293 (2) cell lysate using anti-VIMP antibody at 1/1,000 dilution.
Application
Fig3:ICC staining VIMP (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4:Flow cytometric analysis of Hela cells with VIMP antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).| Positive Control | Human VIMP recombinant protein, VIMP-hIgGFc transfected HEK293 cell lysate, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
抱歉，暂无相关文献
抱歉，暂无浏览记录
