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| Product Name | PARP1 [A0-D11] |
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| Antibody Type | Primary Antibodies |
| Antigen Alias | ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody ADP ribosyltransferase antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody NAD(+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family, member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody poly(ADP ribose) synthetase antibody poly(ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody |
| Product description | Poly(ADP-ribose) polymerase-1 (PARP-1), also designated PARP, is a nuclear DNA-binding zinc finger protein that influences DNA repair, DNA replication, modulation of chromatin structure, and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer of ADP-ribose units from NAD(+) to a number of acceptor molecules including chromatin. PARP-1 recognizes DNA strand interruptions and can complex with RNA and negatively regulate transcription. Actinomycin D- and etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor (AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to chromosomal instability due to higher frequencies of chromosome fusions and aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the efficient maintenance of genome integrity. |
| Immunogen | Peptide |
| Clonality | Monoclonal |
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| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:100 IHC:1:100-1:500 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 2mg/ml |
| Alternative Names | ADP ribosyltransferase (NAD+ poly (ADP ribose) polymerase) antibody ADP ribosyltransferase antibody ADP ribosyltransferase diphtheria toxin like 1 antibody ADP ribosyltransferase NAD(+) antibody ADPRT 1 antibody ADPRT antibody ADPRT1 antibody ARTD1 antibody msPARP antibody NAD(+) ADP ribosyltransferase 1 antibody NAD(+) ADP-ribosyltransferase 1 antibody pADPRT 1 antibody pADPRT1 antibody PARP 1 antibody PARP antibody PARP-1 antibody PARP1 antibody PARP1_HUMAN antibody Poly (ADP ribose) polymerase 1 antibody poly (ADP ribose) polymerase family member 1 antibody Poly [ADP-ribose] polymerase 1 antibody Poly(ADP ribose) polymerase antibody poly(ADP ribose) synthetase antibody poly(ADP ribosyl)transferase antibody Poly[ADP ribose] synthetase 1 antibody Poly[ADP-ribose] synthase 1 antibody PPOL antibody |
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| Molecular Weight(MW) | 113 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of PARP1 on different lysates using anti-PARP1 antibody at 1/100 dilution. Positive control： Lane1: Daudi Lane2: Rat spleen tissue
Application
Fig2: ICC staining PARP1 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- PARP1 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of Daudi cells with PARP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Daudi, rat spleen tissue lysate, rat brain tissue, human tonsil tissue, human pancreas tissue, mouse testis tissue. |
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| Application Notes | WB:1:500 ICC:1:50-1:100 IHC:1:100-1:500 FC:1:50-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
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