| 存储条件 |
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| Product Name | Actin [JJ09-29] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | a actin antibody ACTA antibody ACTA1 antibody Actin alpha skeletal muscle antibody Actin antibody actin, alpha 1, skeletal muscle 1 antibody actin, alpha 1, skeletal muscle antibody Actin, alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody |
| Product description | All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 | |
| Species Reactivity | HumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Alternative Names | a actin antibody ACTA antibody ACTA1 antibody Actin alpha skeletal muscle antibody Actin antibody actin alpha 1 skeletal muscle 1 antibody actin alpha 1 skeletal muscle antibody Actin alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody |
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| Molecular Weight(MW) | 42 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat
Application
Fig2: ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.
Application
Fig7:Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1,000 dilution.Positive control:Lane 1:Hybrid fish (crucian-carp) brain tissueLane 2:Hybrid fish (crucian-carp) kidney tissue| Positive Control | A431, Jurkat, Hela, AGS, NIH/3T3, mouse smooth muscle tissue, mouse cardiac muscle tissue. |
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| Application Notes | WB:1:1,000-1:2,000 ICC:1:100-1:500 IHC:1:50-1:200 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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