| 存储条件 |
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| Product Name | beta Tubulin [SR25-04] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Beta 4 tubulin antibody Beta 5 tubulin antibody BetaTubulin antibody TBB5_HUMAN antibody TUBB antibody TUBB2 antibody TUBB2A antibody TUBB5 antibody tubulin beta 2A antibody Tubulin beta chain antibody Tubulin beta-5 chain antibody |
| Product description | Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin. The beta-tubulin (relative molecular weight about 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer, it is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain. Beta-Tubulin may have bound GTP or GDP. Under certain conditions β-tubulin can hydrolyze its bound GTP to GDP plus Pi, release the Pi, and exchange the GDP for GTP. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Beta 4 tubulin antibody Beta 5 tubulin antibody BetaTubulin antibody TBB5_HUMAN antibody TUBB antibody TUBB2 antibody TUBB2A antibody TUBB5 antibody tubulin beta 2A antibody Tubulin beta chain antibody Tubulin beta-5 chain antibody |
|---|---|
| Molecular Weight(MW) | 50 kDa |
| Cellular Localization | Cytoplasm |
Application
Fig1: Western blot analysis of beta Tubulin on different cell lysates using anti-beta Tubulin antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12
Application
Fig2: ICC staining beta Tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining beta Tubulin in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining beta Tubulin in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: ICC staining beta Tubulin in PC12 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig6: ICC staining beta Tubulin in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Application
Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Application
Fig11: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-beta Tubulin antibody. Counter stained with hematoxylin.
Application
Fig12: Flow cytometric analysis of NIH/3T3 cells with beta Tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | CRC, PC12, Hela, NIH/3T3, N2A, SH-SY-5Y, human kidney tissue, mouse colon tissue, mouse brain tissue, human colon cancer tissue, mouse kidney tissue. |
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| Application Notes | WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:10-1:100 |
| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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