| 存储条件 |
|---|
| Product Name | MMP12 [SR03-23] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 3.4.24.65 antibody HME antibody Macrophage elastase antibody Macrophage metalloelastase antibody Macrophage metaloelastase antibody Matrix metallopeptidase 12 (macrophage elastase) antibody Matrix metalloprotease 12 antibody Matrix metalloproteinase-12 antibody ME antibody MGC138506 antibody MME antibody MMP 12 antibody MMP-12 antibody Mmp12 antibody MMP12_HUMAN antibody |
| Product description | The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-12 (also designated macrophage metalloelastase) is produced in alveolar macrophages and degrades elastin. MMP-12 may contribute to elastin degradation occurring in granulomatous skin diseases and may also participate in macrophage migration through the epidermal and vascular basement membranes in inflammatory disorders. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIPFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | EC 3.4.24.65 antibody HME antibody Macrophage elastase antibody Macrophage metalloelastase antibody Macrophage metaloelastase antibody Matrix metallopeptidase 12 (macrophage elastase) antibody Matrix metalloprotease 12 antibody Matrix metalloproteinase-12 antibody ME antibody MGC138506 antibody MME antibody MMP 12 antibody MMP-12 antibody Mmp12 antibody MMP12_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 54/45/22 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of MMP12 on different cell lysates using anti-MMP12 antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: THP-1
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinomna tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-MMP12 antibody. Counter stained with hematoxylin.
Application
Fig4 Flow cytometric analysis of A549 cells with MMP12 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | A549, MCF-7, Hela, THP-1, human breast carcinomna tissue, mouse lung tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
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