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Myc-Tag (9B11) Mouse mAb (PE Conjugate)

产品编号 : 3739S

英文名称 :

中文名称 : 抗体

品牌 : CST

包装规格	交货周期	质量标准	目录价	会员专享价	数量
100 µl	现货2-3天	原装正品	￥5200	登录后可见	- +

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基础信息产品详情相关文献

基础信息

存储条件	+4℃

产品详情

Product Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry in cells transfected with Myc-tagged protein.

Product Usage Information

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.

Specificity / Sensitivity

Myc-Tag (9B11) Mouse Monoclonal Antibody (PE Conjugate) detects exogenously expressed proteins containing the Myc epitope tag. This antibody conjugate recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody conjugate detects exogenously expressed Myc-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated. This antibody conjugate may cross-react with c-myc protein.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL).

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect, and mammalian cell systems.

Munro, S. and Pelham, H.R. (1984) EMBO J 3, 3087-93.

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