This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Vimentin (D21H3) XP® Rabbit mAb #5741.
Product Usage Information
Application
Dilution
Immunohistochemistry (Paraffin)
1:100 - 1:400
Immunofluorescence (Immunocytochemistry)
1:400
Flow Cytometry (Fixed/Permeabilized)
1:50
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity / Sensitivity
Vimentin (D21H3) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate) detects endogenous levels of total vimentin protein.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein.
