Product Usage Information
| Application | Dilution |
|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 - 1:100 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity / Sensitivity
LC3A/B Antibody detects endogenous levels of total LC3A and LC3B proteins. Cross-reactivity may exist with LC3C. Stronger reactivity is observed with the type II form of LC3A/B.
Species Reactivity:
Human, Mouse, Rat
Species predicted to react based on 100% sequence homology:
Monkey, Chicken, Xenopus, Zebrafish, Dog
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly40 of LC3B. Antibodies are purified by protein A and peptide affinity chromatography.
