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Human Thyroid Stimulating Hormone ELISA Kit (TSH) is a Sandwich (quantitative) ELISA for the measurement of Human Thyroid Stimulating Hormone (TSH) in Human in Biofluids, Cell Culture Media samples.
隐藏别名
Glycoprotein hormones alpha chain, Anterior pituitary glycoprotein hormones common subunit alpha, Choriogonadotropin alpha chain, Chorionic gonadotrophin subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, Luteinizing hormone alpha chain, Lutropin alpha chain, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha, CGA
Colorimetric
Plasma, Cell culture supernatant, Serum
Human
Sandwich (quantitative)
< 4 pg/mL
2.744 - 2000 pg/mL
Microplate
Abcam's TSH Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TSH in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TSH coated on a 96-well plate. Standards and samples are pipetted into the wells and TSH present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TSH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TSH bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
Blue Ice
-20°C
-20°C
-20°C
Representative standard curve using ab100660
Representative standard curve using ab100660
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