Recombinant part protein of Arabidopsis ATCG00190，P50546
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:2500-1:5000
Species Reactivity
ArabidopsisRice
Purification
Affinity purified
Target Information
Gene Symbol
rpoB
Gene Full Name
DNA-directed RNA polymerase subunit beta
Target Description
RPOB encodes chloroplast DNA-dependent RNA polymerase B subunit. It catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
Molecular Weight(MW)
121kDa for Arabidopsis
Application
WB
Sample: Gradient diluted antigen protein Electrophoresis: 10% SDS-PAGE Membrane transfer: NC (nitrocellulose) membrane, voltage 10v overnight Closed: 5% skimmed milk powder, incubated at room temperature for 1 hour Primary antibody: diluted 1:5000, incubated at room temperature for 1 hour
