| 存储条件 |
|---|
| Product Name | CD79a Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human CD79a aa 51-100 / 226. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:50 IF-P:1:50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CD79A |
|---|---|
| Gene Synonyms | IGA MB1 MB-1 IGAlpha |
| Gene Full Name | CD79a molecule |
| Gene Summary | The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins, Ig-alpha and Ig-beta, which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
| Alternative Names | B lymphocyte-specific MB1 protein antibody B-cell antigen receptor complex-associated protein alpha chain antibody CD 79a antibody CD79a antibody CD79a antigen (immunoglobulin-associated alpha) antibody CD79A antigen antibody CD79a molecule immunoglobulin-associated alpha antibody CD79A_HUMAN antibody Ig alpha antibody Ig-alpha antibody B lymphocyte-specific MB1 protein antibody B-cell antigen receptor complex-associated protein alpha chain antibody CD 79a antibody CD79a antibody CD79a antigen (immunoglobulin-associated alpha) antibody CD79A antigen antibody CD79a molecule immunoglobulin-associated alpha antibody CD79A_HUMAN antibody Ig alpha antibody Ig-alpha antibody IGA antibody IgM-alpha antibody Immunoglobulin-associated alpha antibody Ly54 antibody MB-1 membrane glycoprotein antibody MB1 antibody Membrane-bound immunoglobulin-associated protein antibody Surface IgM-associated protein antibody |
| Molecular Weight(MW) | 25kDa(Observed band size:45-55kDa) |
| Cellular Localization | Cell membrane. |
WB
Western blot analysis of CD79a on different lysates with Rabbit anti-CD79a antibody at 1/1,000 dilution. Lane 1: Ramos cell lysate, Lane 2: Daudi cell lysate, Lane 3: Raji cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human spleenr tissue with Rabbit anti-CD79a antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of Daudi cells labeling CD79a with Rabbit anti-CD79a antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD79a antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-P
Species: Human, Site: spleen, Sample: Paraffin-embedded section, Antibody concentration: 1/50.| Application Notes | WB:1:1000 IHC:1:200-1:1000 ICC/IF:1:50 IF-P:1:50 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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