| 存储条件 |
|---|
| Product Name | PELP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human PELP1 aa 1,021-1,070 / 1,130. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:1000 IHC:1:1000 ICC:1:100 IP:1-2μg/sample FC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PELP1 |
|---|---|
| Gene Synonyms | MNAR P160 |
| Gene Full Name | proline, glutamate and leucine rich protein 1 |
| Gene Summary | This gene encodes a transcription factor which coactivates transcription of estrogen receptor responsive genes and corepresses genes activated by other hormone receptors or sequence-specific transcription factors. Expression of this gene is regulated by both members of the estrogen receptor family. This gene may be involved in the progression of several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| Alternative Names | glutamic acid- and leucine-rich protein 1 antibody HMX3 antibody MNAR antibody Modulator of non-genomic activity of estrogen receptor antibody Modulator of nongenomic activity of estrogen receptor antibody P160 antibody Pelp1 antibody PELP1 proline glutamic acid leucine rich protein 1 antibody PELP1_HUMAN antibody proline and glutamic acid rich nuclear protein antibody glutamic acid- and leucine-rich protein 1 antibody HMX3 antibody MNAR antibody Modulator of non-genomic activity of estrogen receptor antibody Modulator of nongenomic activity of estrogen receptor antibody P160 antibody Pelp1 antibody PELP1 proline glutamic acid leucine rich protein 1 antibody PELP1_HUMAN antibody proline and glutamic acid rich nuclear protein antibody Proline glutamate and leucine rich protein 1 antibody Proline- antibody proline- glutamic acid- and leucine-rich protein 1 antibody Transcription factor HMX3 antibody |
| Molecular Weight(MW) | 120kDa(Observed band size: 150kDa) |
| Cellular Localization | Nucleus, nucleolus, nucleoplasm, Cytoplasm. |
WB
Western blot analysis of PELP1 on different lysates with Rabbit anti-PELP1 antibody at 1/1,000 dilution. Lane 1: SW620 cell lysate, Lane 2: MCF7 cell lysate, Lane 3: HeLa cell lysate, Lane 4: HEK-293 cell lysate, Lane 5: NIH/3T3 cell lysate, Lysates/proteins at 20 µg/Lane. Exposure time: Lane 1-4: 10 seconds; Lane 5: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-PELP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of MCF7 cells labeling PELP1 with Rabbit anti-PELP1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PELP1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IP
PELP1 was immunoprecipitated from 0.2 mg HeLa cell lysate with Rabbit anti-PELP1 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-PELP1 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit anti-PELP1 antibody IP in HeLa cell lysate Lane 3: Rabbit IgG instead of Rabbit anti-PELP1 antibody in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes.
FC
Flow cytometric analysis of MCF7 cells labeling PELP1. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:100 IP:1-2μg/sample FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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