| 存储条件 |
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| Product Name | PMP70 Recombinant Rabbit Monoclonal Antibody |
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| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human PMP70 aa 360-659 / 659. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:2000-1:5000 IHC:1:500-1:2000 ICC/IF:1:100 FC:1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ABCD3 |
|---|---|
| Gene Synonyms | ZWS2 ABC43 CBAS5 PMP70 PXMP1 |
| Gene Full Name | ATP binding cassette subfamily D member 3 |
| Gene Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Alternative Names | 70 kDa peroxisomal membrane protein antibody ABC 43 antibody ABC D3 antibody ABC43 antibody ABCD 3 antibody Abcd3 antibody ABCD3 protein antibody ABCD3_HUMAN antibody ATP binding cassette sub family D (ALD) member 3 antibody ATP binding cassette sub family D member 3 antibody 70 kDa peroxisomal membrane protein antibody ABC 43 antibody ABC D3 antibody ABC43 antibody ABCD 3 antibody Abcd3 antibody ABCD3 protein antibody ABCD3_HUMAN antibody ATP binding cassette sub family D (ALD) member 3 antibody ATP binding cassette sub family D member 3 antibody ATP-binding cassette sub-family D member 3 antibody CBAS5 antibody dJ824O18.1 (ATP-binding cassette sub family D (ALD) member 3 (PMP70 PXMP1)) antibody Peroxisomal membrane protein 1 (70kD) antibody Peroxisomal membrane protein 1 (70kD Zellweger syndrome) antibody Peroxisomal membrane protein 1 antibody PMP 70 antibody PMP70 antibody PXMP 1 antibody PXMP1 antibody ZWS2 antibody |
| Molecular Weight(MW) | 76kDa(Observed band size:70kDa) |
| Cellular Localization | Peroxisome membrane. |
WB
Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: A431 cell lysate (20 µg/Lane) Lane 4: Caco-2 cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Lane 6: Human liver tissue lysate (40 µg/Lane) Lane 7: Mouse liver tissue lysate (40 µg/Lane) Lysates/proteins at 10 µg/Lane. Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PMP70 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000-1:5000 IHC:1:500-1:2000 ICC/IF:1:100 FC:1:1000 |
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| Form | Liquid |
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| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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