A synthetic phosphorylated peptide around T357 & S358 & S360 of human MLKL (NP_689862.1).
Modification
p-357/S358/S360
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB:1:500-1:2000
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Symbol
MLKL
Gene Synonyms
hMLKL
Gene Full Name
mixed lineage kinase domain like pseudokinase
Gene Summary
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]
Alternative Names
hMLKL Phospho-MLKL-T357/S358/S360
Molecular Weight(MW)
54kDa
Cellular Localization
Cell membrane, Cytoplasm.
Application
WB
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-MLKL-T357/S358/S360 pAb at 1:1000 dilution. NIH/3T3 cells were treated by TNF-α (20 ng/mL) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG(H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Application Notes
WB:1:500-1:2000
Additional Information
Form
Liquid
Storage Instructions
Store at -20℃. Avoid freeze / thaw cycles.
Storage Buffer
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
