| 存储条件 |
|---|
| Product Name | Kappa Light Chain Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant full protein within human IGKC. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| WB:1:1000 IHC:1:500-1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IGKC |
|---|---|
| Gene Synonyms | Km HCAK1 IGKCD |
| Gene Full Name | immunoglobulin kappa constant |
| Gene Summary | Predicted to enable antigen binding activity. Involved in adaptive immune response. Located in blood microparticle and extracellular exosome. Part of IgG immunoglobulin complex. [provided by Alliance of Genome Resources, Feb 2025] |
| Alternative Names | HCAK 1 antibody HCAK1 antibody Ig kappa chain C region antibody IGKC antibody IGKCD antibody Immunoglobulin InV antibody Immunoglobulin kappa constant antibody Immunoglobulin kappa constant region antibody Immunoglobulin kappa light chain antibody Immunoglobulin kappa light chain constant region antibody HCAK 1 antibody HCAK1 antibody Ig kappa chain C region antibody IGKC antibody IGKCD antibody Immunoglobulin InV antibody Immunoglobulin kappa constant antibody Immunoglobulin kappa constant region antibody Immunoglobulin kappa light chain antibody Immunoglobulin kappa light chain constant region antibody Immunoglobulin KM antibody Kappa 1 immunoglobulin light chain antibody kappa light chain antibody Km antibody MGC111575 antibody MGC62011 antibody MGC72072 antibody MGC88770 antibody MGC88771 antibody MGC88809 antibody |
| Molecular Weight(MW) | 12kDa(Observed band size: 25kDa) |
| Cellular Localization | Cell membrane,Secreted. |
WB
Western blot analysis of Kappa Light Chain on human plasma lysates with Mouse anti-Kappa Light Chain antibody at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Kappa Light Chain antibody at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-Kappa Light Chain antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.| Application Notes | WB:1:1000 IHC:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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