| 存储条件 |
|---|
| Product Name | LAMP1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-FIHCIPWB |
| WB:1:1000 IHC:1:1000 ICC:1:100 IF-F:1:200 IP:1-2μg/sample FC:1:1000 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Lamp1 |
|---|---|
| Gene Synonyms | P2B Perk LGP-A CD107a Lamp-1 LGP-120 |
| Gene Full Name | lysosomal-associated membrane protein 1 |
| Gene Summary | Enables protein domain specific binding activity. Involved in protein stabilization. Located in several cellular components, including cytoplasmic vesicle; sarcolemma; and vacuole. Is active in lysosome. Is expressed in several structures, including central nervous system; craniocervical region bone; egg cylinder; oocyte; and sensory organ. Orthologous to human LAMP1 (lysosomal associated membrane protein 1). [provided by Alliance of Genome Resources, Jan 2025] |
| Alternative Names | CD107 antigen like family member A antibody CD107 antigen-like family member A antibody CD107a antibody CD107a antigen antibody LAMP 1 antibody LAMP-1 antibody LAMP1 antibody LAMP1_HUMAN antibody LAMPA antibody LGP120 antibody CD107 antigen like family member A antibody CD107 antigen-like family member A antibody CD107a antibody CD107a antigen antibody LAMP 1 antibody LAMP-1 antibody LAMP1 antibody LAMP1_HUMAN antibody LAMPA antibody LGP120 antibody lgpA antibody Lysosomal membrane glycoprotein 120KD antibody Lysosomal Associated Membrane Protein 1 antibody Lysosome associated membrane glycoprotein 1 antibody Lysosome-associated membrane glycoprotein 1 antibody Lysosome-associated membrane protein 1 antibody OTTHUMP00000040663 antibody |
| Molecular Weight(MW) | 44kDa(Observed band size: 100-120kDa) |
| Cellular Localization | Lysosome membrane, Endosome membrane, Late endosome membrane, Cell membrane, Cytolytic granule membrane. |
WB
Western blot analysis of LAMP1 on different lysates with Rabbit anti-LAMP1 antibody at 1/1,000 dilution. Lane 1: C2C12 cell lysate, Lane 2: NIH/3T3 cell lysate, Lane 3: RAW264.7 cell lysate, Lane 4: RAW264.7 cell lysate treated with deglycosylation. Lysates/proteins at 30 µg/Lane. Exposure time: 9 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-LAMP1 antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of NIH/3T3 cells labeling LAMP1 with Rabbit anti-LAMP1 antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LAMP1 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.
IF-F
Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-LAMP1 antibody at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
IP
LAMP1 was immunoprecipitated from 0.2 mg NIH/3T3 cell lysate with Rabbit anti-LAMP1 antibody at 2 µg/25 µl agarose. Western blot was performed from the immunoprecipitate using Rabbit anti-LAMP1 antibody at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: NIH/3T3 cell lysate (input), Lane 2: Rabbit anti-LAMP1 antibody IP in NIH/3T3 cell lysate, Lane 3: Rabbit IgG instead of Rabbit anti-LAMP1 antibody in NIH/3T3 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 seconds.
FC
Flow cytometric analysis of NIH/3T3 cells labeling LAMP1. Cells were fixed and permeabilized. Then stained with the primary antibody (1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:1000 IHC:1:1000 ICC:1:100 IF-F:1:200 IP:1-2μg/sample FC:1:1000 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
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