Recombinant human FGF2 protein (Active)

• 产品名称

  重组人FGF2蛋白(Active)
  参阅全部 FGF2 蛋白酶

• 生物活性

  Determined by a cell proliferation assay using Balb/c 3T3 cells.

  The expected ED₅₀ is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg.

• 纯度

  >= 95 % SDS-PAGE.
  >= 95% HPLC Sterile filtered
  

• 内毒素水平

  < 1.000 Eu/µg

• 表达系统

  Escherichia coli

• Accession

  P09038

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Recombinant

• 
  

• 种属

  Human

• 序列

  AAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

• 预测分子量

  17 kDa

• 氨基酸

  135 to 288

Our Abpromise guarantee covers the use of ab9596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Cell Differentiation

  SDS-PAGE

  Cellular Activation

  HPLC

• 形式

  Lyophilized

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  Constituents: 0.0605% Tris, 0.87% Sodium chloride

  This product is an active protein and may elicit a biological response in vivo, handle with caution.

• 复溶

  Reconstitute in 5mM Tris, pH 7.6, to 0.1-1.0 mg/ml.

别名

• Basic fibroblast growth factor

• Basic fibroblast growth factor bFGF

• BFGF

• FGF 2

• FGF B

• FGF-2

• Fgf2

• FGF2 basic

• FGF2_HUMAN

• FGFB

• Fibroblast growth factor 2

• Fibroblast growth factor 2 (basic)

• Fibroblast growth factor, basic

• HBGF 2

• HBGF-2

• HBGF2

• HBGH 2

• HBGH2

• Heparin binding growth factor 2 precursor

• Heparin-binding growth factor 2

• Prostatropin

• 

  Sandwich ELISA - Recombinant human FGF2 protein (ab9596)

  Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1ug/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5ug/ml.

• 

  Cell differentiation - Recombinant human FGF2 protein (ab9596)This image is courtesy of Sun Yung, Centro de Investigación Príncipe Felipe.

  Human embryonic stem cells (H9) were differentiated both as embryoid bodies and monolayer cultures (on Matrigel) using a protocol described in Kennedy et al., 2007. BMP-4, FGF-basic (ab9596) and VEGF (ab9571) were sequentially added to the media to direct differentiation. These images were taken at day 8, where cell adhesion antigens CD31 (ab32457 in green) and CD34 (ab8536 in red) are abundant and represent primitive haematopoietic cells within the culture. Dapi staining is shown in blue.