HRP Anti-GAPDH抗体- Loading Control
参阅全部 GAPDH 一抗
HRP兔多克隆抗体to GAPDH - Loading Control
Rabbit
HRP
适用于: WBmore details
与反应: Human
预测可用于: Mouse, Rat, Cow, Saccharomyces cerevisiae, Xenopus laevis
Full length native protein (purified) corresponding to Human GAPDH.
This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; A431; Hek293.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol, 1% BSA
浓度
100 µl 浓度为 0.5 mg/ml
Protein A purified
This antibody is the HRP conjugated version of ab9485, for more convenient use as a loading control antibody.
多克隆
IgG
Abpromise™承诺保证使用ab9385于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (11) | 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). Milk blocking may cause 'no bands' problem. Please try BSA as well. |
Entrez Gene: 2597 Human
Entrez Gene: 100042025 Mouse
Entrez Gene: 14433 Mouse
Entrez Gene: 380259 Xenopus laevis
Omim: 138400 Human
SwissProt: P04406 Human
SwissProt: P16858 Mouse
SwissProt: P51469 Xenopus laevis
Unigene: 544577 Human
Unigene: 592355 Human
Unigene: 598320 Human
Unigene: 304088 Mouse
Unigene: 309092 Mouse
Unigene: 317779 Mouse
Unigene: 343110 Mouse
Unigene: 392463 Mouse
Unigene: 392480 Mouse
Unigene: 414470 Mouse
Unigene: 458138 Mouse
Unigene: 458416 Mouse
Unigene: 475698 Mouse
Unigene: 129558 Rat
Unigene: 91450 Rat
Unigene: 995 Xenopus laevis
38 kDa BFA-dependent ADP-ribosylation substrate antibody
aging associated gene 9 protein antibody
Aging-associated gene 9 protein antibody
BARS-38 antibody
cb609 antibody
EC 1.2.1.12 antibody
Epididymis secretory sperm binding protein Li 162eP antibody
G3P_HUMAN antibody
G3PD antibody
G3PDH antibody
GAPD antibody
GAPDH antibody
Glyceraldehyde 3 phosphate dehydrogenase antibody
glyceraldehyde 3-PDH antibody
Glyceraldehyde-3-phosphate dehydrogenase antibody
HEL-S-162eP antibody
KNC-NDS6 antibody
MGC102544 antibody
MGC102546 antibody
MGC103190 antibody
MGC103191 antibody
MGC105239 antibody
MGC127711 antibody
MGC88685 antibody
OCAS, p38 component antibody
OCT1 coactivator in S phase, 38-KD component antibody
peptidyl cysteine S nitrosylase GAPDH antibody
Peptidyl-cysteine S-nitrosylase GAPDH antibody
wu:fb33a10 antibody
Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)
All lanes : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 38 kDawhy is the actual band size different from the predicted?
Exposure time: 1 minute
Lane 1 - 4 : GAPDH antibody - Loading Control (ab9385) at 1 ug/ml
Lane 1 : HeLa cell lysate at 20 ug
Lane 2 : A431 cell lysate at 20 ug
Lane 3 : Jurkat cell lysate at 20 ug
Lane 4 : 293 cell lysate at 20 ug
Performed under reducing conditions.
Exposure time : 1 minute
Western blot - HRP Anti-GAPDH antibody - Loading Control (ab9385)
Lanes 1-4 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% BSA))
Lanes 5-8 : HRP Anti-GAPDH antibody - Loading Control (ab9385) at 1/5000 dilution ((Blocked in 5% MILK))
Lanes 1 & 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252)
Lanes 2 & 6 : Jurkat (Human) Whole Cell Lysate (ab52254)
Lanes 3 & 7 : A-431 whole cell lysate (ab7909)
Lanes 4 & 8 : HEK-293 whole cell lysate (ab7902)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
The membrane 1-4 was blocked in 5% BSA (1 hour). The membrane 5-8 was blocked in 5% MILK (1 hour). Abcam routinely uses 5% BSA to block, however following recent customer feedback our labs investigated the effect of 5% milk blocking. We can now confirm that milk is not a suitable blocking agent for this antibody and significantly decreases the signal on the membrane.
