Anti-ORC4L抗体
参阅全部 ORC4L 一抗
山羊多克隆抗体to ORC4L
Goat
适用于: WBmore details
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog
Synthetic peptide corresponding to Human ORC4L aa 400-500 (C terminal). (NP_001177810.1; NP_001177811.1)
Database link: NP_002543.2
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
浓度
100 µg 浓度为 0.5 mg/ml
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
多克隆
IgG
Abpromise™承诺保证使用ab9641于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 1 hour primary incubation is recommended for this product. |
Entrez Gene: 5000 Human
Entrez Gene: 26428 Mouse
Omim: 603056 Human
SwissProt: O43929 Human
SwissProt: O88708 Mouse
Unigene: 558364 Human
Unigene: 291059 Mouse
Unigene: 314338 Mouse
Unigene: 144844 Rat
Origin recognition complex, subunit 4, S. cerevisiae, homolog of antibody
FLJ46668 antibody
HSORC4 antibody
ORC 4 antibody
ORC 4L antibody
ORC 4P antibody
ORC4 antibody
ORC4_HUMAN antibody
ORC4L antibody
ORC4L protein antibody
ORC4P antibody
Origin recognition complex subunit 4 (yeast homolog) like antibody
Origin recognition complex subunit 4 antibody
Origin recognition complex subunit 4 like (yeast) antibody
Origin recognition complex subunit 4 like antibody
origin recognition complex, subunit 4 homolog antibody
Origin recognition complex, subunit 4, S. cerevisiae, homolog-like antibody
Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)
Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg
Predicted band size: 50 kDa
Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)
Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)
ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
Western blot - Anti-ORC4L antibody - ChIP Grade (ab9641)
All lanes : Anti-ORC4L antibody (ab9641) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDawhy is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
