Anti-Phosphoserine抗体
参阅全部 Phosphoserine 一抗
兔多克隆抗体to Phosphoserine
Rabbit
Recognize proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. Will detect 50 ng of phosvitin with immunoblotting or 0.5 ng of phosvitin with immunocaptured ELISA. Antibody slightly cross-reacts with phosphothreonine (about 20%) based on indirect ELISA data.
适用于: WBmore details
与反应: Species independent
Chemical/ Small Molecule corresponding to Phosphoserine conjugated to keyhole limpet haemocyanin. KLH-phosphoserine conjugate and phosvitin mixture.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 6.00
Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.9% PBS
浓度
100 µg 浓度为 0.25 mg/ml
Immunogen affinity purified
多克隆
IgG
Abpromise™承诺保证使用ab9332于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (7) | Use a concentration of 2 - 4 µg/ml. To block use 3%BSA with 0.1% gelatin (do not use milk). We recommend that the antibody solution should contain 0.5% BSA to prevent non-specific binding. |
phospho-Ser antibody
pS antibody
pSER antibody
Western blot - Anti-Phosphoserine antibody (ab9332)This image is courtesy of an Abreview submitted by Karin Birkenkamp-Demtroeder
All lanes : Anti-Phosphoserine antibody (ab9332) at 3 µg/ml (in PBS for 16 hours)
All lanes : Whole cell lysate of monkey COS7 cells
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : An HRP-conjugated Pig anti-rabbit IgG polyclonal at 1/3000 dilution
Blocking Step: 10% Milk for 1 hour at room temperature
Western blot - Anti-Phosphoserine antibody (ab9332)
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies
The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor.
The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E). Actin level was measured as an internal standard of cell protein.
Western blotting of a melanoma cell lysate with anti-phosphoserine antibodies. The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid (lane E). Actin level was measured as an internal standard of cell protein.
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