Anti-BRCA1 antibody

• 产品名称

  Anti-BRCA1抗体
  参阅全部 BRCA1 一抗

• 描述

  兔多克隆抗体to BRCA1

• 宿主

  Rabbit

• 经测试应用

  适用于: IP, WBmore details

• 种属反应性

  与反应: Human
  预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant

• 免疫原

  Synthetic peptide (Human) - which represents aportion within the last 100 amino acids of the human Breast Cancer Gene 1 (GenBank PID 627392), conjugated to KLH.

• 阳性对照

• WB: HEK-293T whole cell lysate. IP: HEK293-T whole cell lysate.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液

  pH: 7
  Preservative: 0.1% Sodium azide
  Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris

• 浓度

• 50 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 纯化说明

  Antibodies were affinity purified using thepeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab9141于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 672 Human

• Entrez Gene: 712634 Rhesus monkey

• Omim: 113705 Human

• SwissProt: Q9GKK8 Chimpanzee

• SwissProt: Q6J6I8 Gorilla

• SwissProt: P38398 Human

• SwissProt: Q6J6I9 Rhesus monkey

• Unigene: 194143 Human

• 别名

• BRCA1/BRCA2 containing complex subunit 1 antibody

• BRCA1/BRCA2-containing complex, subunit 1 antibody

• BRCA1_HUMAN antibody

• BRCAI antibody

• BRCC 1 antibody

• BRCC1 antibody

• Breast and ovarian cancer susceptibility protein 1 antibody

• Breast Cancer 1 antibody

• Breast Cancer 1 Early Onset antibody

• Breast cancer type 1 susceptibility protein antibody

• BROVCA1 antibody

• FANCS antibody

• IRIS antibody

• PNCA4 antibody

• PPP1R53 antibody

• Protein phosphatase 1 regulatory subunit 53 antibody

• PSCP antibody

• RING finger protein 53 antibody

• RNF53 antibody

• BRCA 1 antibody

• BRCA1 antibody

• BRCA1 DNA repair associated antibody

• 

  Western blot - Anti-BRCA1 antibody (ab9141)

  Anti-BRCA1 antibody (ab9141) at 0.04 µg/ml + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer) at 50 µg
  
  Predicted band size: 220 kDa
  
  
  Exposure time: 30 seconds
  

• 

  Immunoprecipitation - Anti-BRCA1 antibody (ab9141)

  Detection of BRCA1 by immunoprecipitation of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

  Lane 1: Anti-BRCA1 antibody (ab9141) used for IP at 6 µg per reaction.

  Lane 2: IgG Control

  For blotting immunoprecipitated BRCA1, ab9141 was used at 0.04 µg/mL.

  Detection: Chemiluminescence with an exposure time of 75 seconds.