Anti-SMC1A抗体
参阅全部 SMC1A 一抗
兔多克隆抗体to SMC1A
Rabbit
适用于: IHC-P, IP, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Cow
Synthetic peptide within Human SMC1A aa 1133-1233 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q14683
WB: HeLa, 293T and NIH3T3 whole cell lysate. IHC-P: Human ovarian cancer tissue, mouse colon cancer tissue CT26. IP: HeLa whole cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
浓度
50 µg 浓度为 1 mg/ml
Immunogen affinity purified
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG.
多克隆
IgG
Abpromise™承诺保证使用ab9262于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IP | Use at 1-4 µg/mg of lysate. | |
| WB | (5) | 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa). |
Entrez Gene: 8243 Human
Entrez Gene: 24061 Mouse
Omim: 300040 Human
SwissProt: Q14683 Human
SwissProt: Q9CU62 Mouse
Unigene: 211602 Human
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Western blot - Anti-SMC1A antibody (ab9262)
All lanes : Anti-SMC1A antibody (ab9262) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 seconds
Detection: Chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse colon cancer tissue CT26, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.
Immunoprecipitation - Anti-SMC1A antibody (ab9262)
SMC1A was immunoprecipitated from 1 mg HeLa whole cell lysate with ab9262 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243875 at 1/1000 dilution.
Lysates prepared using NETN lysis buffer.
Lane 1: ab9262 IP in HeLa whole cell lysate.
Lane 2: Contol IgG in HeLa whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
