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Anti-Cytokeratin 19 antibody [RCK108]

产品编号 : ab9221

英文名称 :

中文名称 : 抗体

产品等级 :

品牌 : Abcam

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产品详情

产品名称
Anti-Cytokeratin 19抗体[RCK108]
参阅全部 Cytokeratin 19 一抗
描述
小鼠单克隆抗体[RCK108] to Cytokeratin 19
宿主
Mouse
特异性
This antibody reacts exclusively with cytokeratin 19 which is present in glandular-type epithelia and most carcinomas. It does not react with hepatocytes and hepatocellular carcinoma.
经测试应用
适用于: Flow Cyt, IHC-Fr, IHC-Pmore details
种属反应性
与反应: Human, Zebrafish
免疫原
Tissue, cells or virus corresponding to Human Cytokeratin 19. Human bladder carcinoma cell line T24.
常规说明

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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形式
Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS
纯度
Protein A purified
Primary antibody说明
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
克隆
单克隆
克隆编号
RCK108
骨髓瘤
Sp2/0-Ag14
同种型
IgG1
The Abpromise guarantee
Abpromise™承诺保证使用ab9221于以下的经测试应用
“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
应用 Ab评论说明
Flow Cyt
1/100 - 1/200.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr (1)
1/100 - 1/200.
IHC-P (1)
1/100 - 1/200.
数据库链接

别名

应用	Ab评论	说明
Flow Cyt		1/100 - 1/200. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr	(1)	1/100 - 1/200.
IHC-P	(1)	1/100 - 1/200.

CK19 antibody
Cytokeratin 19 antibody
Cytokeratin-19 antibody
K19 antibody
K1C19_HUMAN antibody
K1CS antibody
Keratin 19 antibody
Keratin type I 40 kD antibody
Keratin type I 40kD antibody
Keratin type I cytoskeletal 19 antibody
Keratin, type I cytoskeletal 19 antibody
Keratin, type I, 40 kd antibody
Keratin-19 antibody
KRT19 antibody
MGC15366 antibody
40 kDa keratin intermediate filament antibody
CK 19 antibody
CK-19 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Immunohistochemistry on a frozen tissue sections of human kidney with ab9221 (1:500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human small intestine paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Immunohistochemistry on frozen tissue sections of human colon with ab9221 (1:500).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Indirect immunoperoxidase staining of human placenta paraffin tissue section with ab9221. Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
ab9221 staining Cytokeratin 19 in human skin (sweat glands).
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Flow Cytometry - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Overlay histogram showing MCF-7 cells stained with ab9221 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9221, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
ab9221 staining cytokeratin 19 in three-day-old Zebrafish tissue by Immunohistochemistry (Frozen sections).
Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 19 antibody [RCK108] (ab9221)
Immunohistochemistry on frozen section of human liver bile duct epithelium