Anti-Transferrin Receptor antibody [MEM-75]

• 产品名称

  Anti-Transferrin Receptor抗体[MEM-75]
  参阅全部 Transferrin Receptor 一抗

• 描述

  小鼠单克隆抗体[MEM-75] to Transferrin Receptor

• 宿主

  Mouse

• 特异性

  Human CD71 (transferrin receptor). This antibody does not block the binding of transferrin to the receptor.

• 经测试应用

  适用于: Flow Cyt, ICC/IFmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Tissue, cells or virus corresponding to Human Transferrin Receptor. Pre-B cell line NALM-6

• 阳性对照

• This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: DU145 and HeLa

• 常规说明

  This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 8.0
  Preservative: 0.097% Sodium azide
  Constituent: PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Protein A purified

• 纯化说明

  Purified from TCS. Purity >95% by SDS-PAGE.

• 克隆

  单克隆

• 克隆编号

  MEM-75

• 骨髓瘤

  unknown

• 同种型

  IgG1

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab9179于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 7037 Human

• Omim: 190010 Human

• SwissProt: P02786 Human

• Unigene: 529618 Human

• 别名

• IMD46 antibody

• OTTHUMP00000208523 antibody

• OTTHUMP00000208524 antibody

• OTTHUMP00000208525 antibody

• p90 antibody

• sTfR antibody

• T9 antibody

• TFR 1 antibody

• TfR antibody

• TfR1 antibody

• TFR1_HUMAN antibody

• TFRC antibody

• TR antibody

• Transferrin receptor (p90 CD71) antibody

• Transferrin receptor protein 1, serum form antibody

• Trfr antibody

• CD 71 antibody

• CD71 antibody

• CD71 antigen antibody

• 

  Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-75] (ab9179)

  Flow cytometric analysis of Human PHA stimulated Peripheral Blood cells labelling Transferrin Receptor with ab9179 at 0.56 ug/ml showing separation of human CD3 positive CD71 positive lymphocytes (red-filled) from CD3 negative CD71 negative lymphocytes (black-dashed).

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [MEM-75] (ab9179)

  Immunocytochemistry staining of CD71 in human HeLa cell line using anti-CD71 (green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

• 

  Flow Cytometry - Anti-Transferrin Receptor antibody [MEM-75] (ab9179)

  Overlay histogram showing Jurkat cells stained with ab9179 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9179, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a diminished signal in Jurkat cells fixed with methanol (5 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Transferrin Receptor antibody [MEM-75] (ab9179)

  ab9179 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab9179 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.