Native人Collagen III蛋白
参阅全部 Collagen III 蛋白酶
Native
Full length protein
No
Native
Human
Our Abpromise guarantee covers the use of ab7535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用
Western blot
SDS-PAGE
Immunoprecipitation
ELISA
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Sodium azide
Constituent: 3% Acetic acid
Alpha 1 type III collagen
Alpha1 (III) collagen
CO3A1_HUMAN
COL 3A1
COL3A1
Collagen alpha 1(III) chain
Collagen alpha-1(III) chain
Collagen III alpha 1 chain precursor
Collagen III alpha 1 polypeptide
Collagen type III alpha
Collagen type III alpha 1
Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant)
Collagen type III alpha 1 chain
Collagen, fetal
EDS4A
Ehlers Danlos syndrome type IV, autosomal dominant
Fetal collagen
Type III collagen
Western blot - Native Human Collagen III protein (ab7535)Image courtesy of Michaela Leyh by Abreview.
All lanes : Anti-Collagen III antibody [FH-7A] (ab6310) at 1/500 dilution
Lane 1 : plus ab7535 reduced and denatured
Lane 2 : plus ab7535 native
Secondary
All lanes : Goat anti-mouse-HRP at 1/2000 dilution
Predicted band size: 139 kDa
The protein can be used reduced + denaturated (band size ~130 kDa) or native (two bands ~ 130kDa and ~300kDa) The native protein gives a brighter signal.
PH of the protein lysate is low - a loading dye with bromphenolblue changes its colour. This does not effect the western blot.
SDS-PAGE - Native Human Collagen III protein (ab7535)
SDS-PAGE analysis of ab7535.
Lane 1: ab7535 Reduced 1 µg.
Lane 2: Pre-stained Molecular Weight Marker.
Lane 3: ab7535 Non-Reduced 1 µg.
