Native Human Collagen I protein

• 产品名称

  Native人Collagen I蛋白
  参阅全部 Collagen I 蛋白酶

• 表达系统

  Native

• Accession

  P02452

• 蛋白长度

  Full length protein

• 无动物成分

  No

• 性质

  Native

• 
  

• 种属

  Human

• 预测分子量

  139 kDa

Our Abpromise guarantee covers the use of ab7533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

• 应用

  Western blot

  SDS-PAGE

• 形式

  Liquid

• 补充说明

  This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins. This product reacts with anti-Collagen Type I. Reaction with anti-Collagen II, III, IV, V or VI is negligible (typically less than 1% cross reactivity was detected by ELISA).

• 浓度

• 500 µg 浓度为 1 mg/ml

• 稳定性和存储

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  Preservative: 0.01% Sodium azide
  Constituent: 3% Acetic acid

• 别名

• Alpha 1 type I collagen

• Alpha 2 type I collagen

• alpha 2 type I procollagen

• alpha 2(I) procollagen

• alpha 2(I)-collagen

• Alpha-1 type I collagen

• alpha1(I) procollagen

• CO1A1_HUMAN

• COL1A1

• COL1A2

• collagen 1

• collagen alpha 1 chain type I

• Collagen alpha-1(I) chain

• collagen alpha-1(I) chain preproprotein

• Collagen I alpha 1 polypeptide

• Collagen I alpha 2 polypeptide

• collagen of skin, tendon and bone, alpha-1 chain

• collagen of skin, tendon and bone, alpha-2 chain

• Collagen type I alpha 1

• Collagen type I alpha 2

• EDSC

• OI1

• OI2

• OI3

• OI4

• pro-alpha-1 collagen type 1

• type I proalpha 1

• Type I procollagen

• type I procollagen alpha 1 chain

• 

  Western blot - Native Human Collagen I protein (ab7533)

  Anti-Collagen I + Collagen III antibody (ab34710) at 1/1000 dilution + Native Human Collagen I protein (ab7533) at 0.05 µg
  
  Secondary
  DyLight™ 649 Rabbit Secondary Antibody at 1/20000 dilution
  
  Observed band size: 130 kDawhy is the actual band size different from the predicted?
  
  
  
  

  DyLight™ 649 anti-rabbit secondary antibody at 1:20,000 for 30 min at RT.

  Blocking Buffer for 30 min at room temperature - proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

  Other Band(s): Collagen Type I splice variants and isoforms.