Anti-Sprouty 4/Spry-4抗体
参阅全部 Sprouty 4/Spry-4 一抗
兔多克隆抗体to Sprouty 4/Spry-4
该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。Read the terms of use »
Rabbit
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity is expected with Sprouty-4.
与反应: Mouse, Human
预测可用于: Cow, Chimpanzee
Synthetic peptide corresponding to Human Sprouty 4/Spry-4 aa 283-299 conjugated to keyhole limpet haemocyanin.
Sequence:
CKAASGDAKTSRPDKPF
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 1.1 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human Sprouty-4 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).Fast track terms of use
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | ||
| WB | (1) |
应用说明
Tested in peptide ELISA.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested by Western blot against cell lysates. We believe that Sprouty 4 is represented by the doublet at ~ 35kDa. We believe that this protein may require IP before Western blotting, corresponding to results seen in Sasaki et al. We have not yet succeeded in peptide blocking the bands detected with this antibody.
Entrez Gene: 81848 Human
Entrez Gene: 24066 Mouse
Omim: 607984 Human
SwissProt: Q9C004 Human
SwissProt: Q9WTP2 Mouse
Unigene: 323308 Human
Unigene: 42038 Mouse
Protein sprouty homolog 4 antibody
Sprouty 4 antibody
Sprouty homolog 4 antibody
sprouty homolog 4 (Drosophila) antibody
SPRY 4 antibody
Spry-4 antibody
Spry4 antibody
SPY4_HUMAN antibody
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)
Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.
Western blot using ab7513 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: 293
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 2minutes.
Lysates at 5µg per lane.
We believe that CTCF is one of the bands seen in the pair of bands between 25 and 37kD.
Western blot - Anti-Sprouty 4/Spry-4 antibody (ab7513)
All lanes : Anti-Sprouty 4/Spry-4 antibody (ab7513) at 1/100 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : SW13 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
The blot was incubated with ab7516 (1/100) overnight at 4°C.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)
ab7513 at 2.5 µg/ml staining human heart tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sprouty 4/Spry-4 antibody (ab7513)
ab7513 staining tumour cells in human liver tissue. Brown color indicates presence of protein, blue color shows cell nuclei.
