Anti-CD4抗体[W3/25]
参阅全部 CD4 一抗
小鼠单克隆抗体[W3/25] to CD4
Mouse
适用于: Flow Cytmore details
不适用于: IHC-P
与反应: Rat
Tissue, cells or virus corresponding to Rat CD4. Rat thymocyte membrane.
Database link: P05540
Flow cyt: Lewis rat splenocytes.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
10 µg 浓度为 1 mg/ml
100 µg 浓度为 1 mg/ml
Affinity purified
单克隆
W3/25
IgG1
kappa
Abpromise™承诺保证使用ab6413于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use a concentration of 0.2 µg/ml. |
应用说明
Is unsuitable for IHC-P.
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![Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)](https://www.abcam.cn/ps/products/6/ab6413/Images/ab6413-1-ab6413antiratCD4spleensplenocytesflowcytometry.jpg)
Flow Cytometry - Anti-CD4 antibody [W3/25] (ab6413)
Lewis rat splenocytes stained with ab6413 (right) or mouse IgG1κ (ab170190) isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab6413) or mouse IgG1κ (ab170190) (1x106 in 100 µl at 0.2 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150117) was used at 1/2000 dilution for 30 min on ice. The cells were simultaneously stained with CD3 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
