Anti-Egr1抗体
参阅全部 Egr1 一抗
兔多克隆抗体to Egr1
该产品是 快线抗体，通过亲和纯化得到。对抗原肽有高灵敏度及特异性（ELISA测试）。Read the terms of use »
Rabbit
A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee sources based on 100% homology for the immunogen sequence. This antibody is expected to cross react with EGR-1.
与反应: Mouse, Human
预测可用于: Rat
Synthetic peptide:
GEQPYEHLTAESFPDI-C
conjugated to KLH, corresponding to amino acids 93-108 of Human Egr1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride
浓度
100 µg 浓度为 0.93 mg/ml
Immunogen affinity purified
This affinity purified antibody is directed against human EGR-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
多克隆
IgG
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).Fast track terms of use
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) | |
| IHC-P |
应用说明
Tested in peptide ELISA.
We have detected a band of 58kDa in mouse embryonic fibroblast whole cell lysate, but we have failed to detect a band at 83kDa (expected size from data from other antibodies). We would appreciate any customer feedback for this antibody.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Entrez Gene: 1958 Human
Entrez Gene: 13653 Mouse
Omim: 128990 Human
SwissProt: P18146 Human
SwissProt: P08046 Mouse
Unigene: 326035 Human
Unigene: 708393 Human
Unigene: 181959 Mouse
Unigene: 9096 Rat
AT225 antibody
Early growth response 1 antibody
Early growth response protein 1 antibody
EGR 1 antibody
EGR-1 antibody
EGR1 antibody
EGR1_HUMAN antibody
G0S30 antibody
KROX 24 antibody
KROX24 antibody
Nerve growth factor-induced clone A antibody
Nerve growth factor-induced protein A antibody
NGFI-A antibody
NGFIA antibody
TIS8 antibody
Transcription factor ETR103 antibody
Transcription factor Zif268 antibody
ZIF 268 antibody
ZIF268 antibody
Zinc finger protein 225 antibody
Zinc finger protein Krox-24 antibody
ZNF225 antibody
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
Western blot - Anti-Egr1 antibody (ab6054)
Anti-Egr1 antibody (ab6054) at 1/1500 dilution + Mouse embryonic fibroblast whole cell lysate at 35 µg
Secondary
Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 58 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Egr1 antibody (ab6054)
ab6054 at 10 µg/ml staining human ovarian cancinoma.
