Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1]

• 产品名称

  Anti-Muscarinic Acetylcholine Receptor 2/CM2抗体[31-1D1]
  参阅全部 Muscarinic Acetylcholine Receptor 2/CM2 一抗

• 描述

  小鼠单克隆抗体[31-1D1] to Muscarinic Acetylcholine Receptor 2/CM2

• 宿主

  Mouse

• 特异性

  This antibody is specific for the m2 mAChR subtype.

• 经测试应用

  适用于: WB, ICC/IF, IHC-Pmore details

• 种属反应性

  与反应: Mouse, Rat, Human
  不与反应: Chicken

• 免疫原

  Full length native protein (purified) corresponding to Pig Muscarinic Acetylcholine Receptor 2/CM2. Purified from Pig Heart.

• 阳性对照

• WB: Human brain and spinal cord tissue lysate. Mouse brain tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC12 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituent: 0.1% BSA

• 纯度

  Protein A purified

• 克隆

  单克隆

• 克隆编号

  31-1D1

• 同种型

  IgG1

The Abpromise guarantee

Abpromise™承诺保证使用ab2805于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 1129 Human

• Entrez Gene: 243764 Mouse

• Entrez Gene: 81645 Rat

• Omim: 118493 Human

• SwissProt: P08172 Human

• SwissProt: Q9ERZ4 Mouse

• SwissProt: P10980 Rat

• Unigene: 535891 Human

• Unigene: 448632 Mouse

• Unigene: 10752 Rat

• Unigene: 200974 Rat

别名

• 7TM receptor antibody

• Acetylcholine receptor, muscarinic, 2 antibody

• AChR antibody

• Acm2 antibody

• ACM2_HUMAN antibody

• Cholinergic receptor muscarinic 2 antibody

• Cholinergic receptor, muscarinic 2, cardiac antibody

• Cholinergic receptor, muscarinic 2, isoform a antibody

• Cholinergic receptor, muscarinic 2a antibody

• CHRM 2 antibody

• CHRM2 antibody

• chrm2a antibody

• CM2 antibody

• FLJ43243 antibody

• HM 2 antibody

• HM2 antibody

• M2 antibody

• M2 muscarinic receptor antibody

• M2-mAChR antibody

• MGC120006 antibody

• MGC120007 antibody

• Muscarinic acetylcholine receptor M2 antibody

• Muscarinic M2 receptor antibody

• 

  Western blot - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)

  All lanes : Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805) at 1 µg/ml
  
  Lane 1 : Human brain tissue lysate - total protein (ab29466)
  Lane 2 : Human spinal cord tissue lysate - total protein (ab29188)
  Lane 3 : Brain (Mouse) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Predicted band size: 52 kDa
  Observed band size: 64 kDawhy is the actual band size different from the predicted?
  Additional bands at: 22 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
  
  
  Exposure time: 30 seconds
  
  
  

  Muscarinic Acetylcholine Receptor 2/CM2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)

  Immunohistochemistry was performed on normal biopsies of deparaffinized human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer and microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a Mouse monoclonal antibody recognizing Muscarinic Acetylcholine Receptor 2/CM2 (ab2805) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Muscarinic Acetylcholine Receptor 2/CM2 antibody [31-1D1] (ab2805)

  ICC/IF image of ab2805 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2805, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.