Anti-GRK1 antibody [D11]

• 产品名称

  Anti-GRK1抗体[D11]
  参阅全部 GRK1 一抗

• 描述

  小鼠单克隆抗体[D11] to GRK1

• 宿主

  Mouse

• 特异性

  Detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.

• 经测试应用

  适用于: ICC/IFmore details

• 种属反应性

  与反应: Mouse, Cow, Human
  

• 免疫原

  Full length native protein (purified) corresponding to Human GRK1.

• 表位

  This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide

• 纯度

  Protein G purified

• Primary antibody说明

  The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

• 克隆

  单克隆

• 克隆编号

  D11

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2776于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

数据库链接

• Entrez Gene: 131890 Human

• Entrez Gene: 6011 Human

• Entrez Gene: 24013 Mouse

• Omim: 180381 Human

• SwissProt: Q15835 Human

• SwissProt: Q8WTQ7 Human

• SwissProt: Q9WVL4 Mouse

• Unigene: 103501 Human

• Unigene: 680654 Human

• Unigene: 721727 Human

• Unigene: 257501 Mouse

别名

• EC=2.7.11.14 antibody

• G protein-coupled receptor kinase 1 antibody

• GPRK1 antibody

• Grk1 antibody

• Rhodopsin kinase antibody

• RHOK antibody

• RK antibody

• RK_HUMAN antibody

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

  Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

  Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:200) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

  Immunocytochemistry/Immunofluorescence analysis of GRK1 (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab2776 (1:20) overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-GRK1 antibody [D11] (ab2776)

  Immunolocalization of GRK1 in bovine retina using ab2776.