Anti-Collagen I抗体[COL-1]
参阅全部 Collagen I 一抗
小鼠单克隆抗体[COL-1] to Collagen I
Mouse
适用于: IHC-Fr, WBmore details
与反应: Human
预测可用于: Rat, Cow, Pig, Deer
Full length native protein (purified) corresponding to Cow Collagen I. Cow skin collagen type I
The epitope recognized by the antibody may be sensitive to routine formalin fixation and paraffin embedding. There have been varying results when using this antibody in IHC-P. Please refer to our customer Abreviews for more protocol information and optimization steps when using this antibody in IHC-P.
WB: Natural Cow Collagen I protein (ab7526), IHC-Fr: Human tonsil tissue.
Production of this antibody has been changed on 23rd June 2016. The following lots are from ascites and are still in stock as of 23rd June 2016 : GR210978, GR175242, GR158374. Lot numbers higher than GR210978 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.0976% Sodium azide
Constituent: PBS
浓度
批次浓度范围 50 µl 浓度为 1.2 - 1.3 mg/ml
Proprietary Purification
Purified from Tissue culture supernatant.
单克隆
COL-1
IgG1
Abpromise™承诺保证使用ab6308于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (2) | Use a concentration of 3.5 - 7 µg/ml. (amplification required).Use on unfixed tissue or acetone fixed tissue. |
| WB | (2) | Use a concentration of 1 - 2 µg/ml. Use under non reducing condition. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa). The antibody is reactive with the native (non-denaturing, helical) form of collagen type I and not reactive when tested on thermally denatured molecules. Use native (non-denaturing) conditions. Positive Control: Hu stomach, skin and adrenal gland tissue lysates. Acid or enzyme treatment with pepsin is a better method to isolate collagen. Continuous refrigeration throughout collagen extraction is important to avoid degradation and denaturation. Take care with pH, temperature, and concentration to avoid collagen polymerization. Read More |
Entrez Gene: 1277 Human
Entrez Gene: 1278 Human
Omim: 120150 Human
Omim: 120160 Human
SwissProt: P02452 Human
SwissProt: P08123 Human
Unigene: 172928 Human
Unigene: 681002 Human
Unigene: 2953 Rat
Alpha 1 type I collagen antibody
Alpha 2 type I collagen antibody
alpha 2 type I procollagen antibody
alpha 2(I) procollagen antibody
alpha 2(I)-collagen antibody
Alpha-1 type I collagen antibody
alpha1(I) procollagen antibody
CO1A1_HUMAN antibody
COL1A1 antibody
COL1A2 antibody
collagen 1 antibody
collagen alpha 1 chain type I antibody
Collagen alpha-1(I) chain antibody
collagen alpha-1(I) chain preproprotein antibody
Collagen I alpha 1 polypeptide antibody
Collagen I alpha 2 polypeptide antibody
collagen of skin, tendon and bone, alpha-1 chain antibody
collagen of skin, tendon and bone, alpha-2 chain antibody
Collagen type I alpha 1 antibody
Collagen type I alpha 2 antibody
EDSC antibody
OI1 antibody
OI2 antibody
OI3 antibody
OI4 antibody
pro-alpha-1 collagen type 1 antibody
type I proalpha 1 antibody
type I procollagen alpha 1 chain antibody
Type I procollagen antibody
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.cn/ps/products/6/ab6308/Images/ab6308-12223-anti-collagen-i-antibody-col-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)This image was kindly supplied as part of the review submitted by Arvi-Matti Kuusniemi
Immunoperoxidase staining of unfixed frozen tissue sections with ab6308. Picture of human kidney cortex showing two glomeruli and surrounding tubulointerstitium.
![Western blot - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.cn/ps/products/6/ab6308/Images/ab6308-337140-anti-collagen-i-antibody-col-1-western-blot.jpg)
Western blot - Anti-Collagen I antibody [COL-1] (ab6308)
Lane 1 : Anti-Collagen I antibody [COL-1] (ab6308) at 2 µg/ml
Lane 2 : Anti-Collagen I antibody [COL-1] (ab6308) at 1 µg/ml
Lane 3 : Anti-Collagen I antibody [COL-1] (ab6308) at 0.5 µg/ml
Lane 4 : Anti-Collagen I antibody [COL-1] (ab6308) at 0 µg/ml
All lanes : Recombinant human Collagen 1
Predicted band size: 130 kDa
![Western blot - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.cn/ps/products/6/ab6308/Images/ab6308-12219-anti-collagen-i-antibody-col-1-western-blot.jpg)
Western blot - Anti-Collagen I antibody [COL-1] (ab6308)This image is courtesy of an anonymous Abreview
Anti-Collagen I antibody [COL-1] (ab6308) at 1/1000 dilution + Human brain whole tissue lysate at 10 µg
Secondary
An HRP-conjugated goat polyclonal to mouse IgG at 1/7500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 130 kDa
Exposure time: 24 hours
![Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)](https://www.abcam.cn/ps/products/6/ab6308/Images/ab6308-337141-anti-collagen-i-antibody-col-1-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [COL-1] (ab6308)
Acetone-fixed human tonsil tissue frozen section stained for Collagen I with ab6308 at 7 μg/mL in immunohistochemical analysis.
