Anti-Dnmt3a antibody

• 产品名称

  Anti-Dnmt3a抗体
  参阅全部 Dnmt3a 一抗

• 描述

  兔多克隆抗体to Dnmt3a

• 宿主

  Rabbit

• 特异性

  This antibody detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1.

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Mouse, Human
  

• 免疫原

  Synthetic peptide corresponding to Human Dnmt3a aa 50-150.

  Run BLAST with  Run BLAST with 

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.05% Sodium azide
  Constituents: 0.1% BSA, 99% PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2850于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 1788 Human

• Entrez Gene: 13435 Mouse

• Omim: 602769 Human

• SwissProt: Q9Y6K1 Human

• SwissProt: O88508 Mouse

• Unigene: 515840 Human

• Unigene: 5001 Mouse

• 别名

• DNA cytosine methyltransferase 3A2 antibody

• DNA methyltransferase 3 alpha antibody

• DNA methyltransferase 3a antibody

• DNA methyltransferase HsaIIIA antibody

• DNA MTase HsaIIIA antibody

• DNM3A_HUMAN antibody

• DNMT 3a antibody

• DNMT antibody

• Dnmt3a antibody

• DNMT3A2 antibody

• M.HsaIIIA antibody

• MCMT antibody

• OTTHUMP00000201149 antibody

• TBRS antibody

• DNA (cytosine 5) methyltransferase 3 alpha antibody

• DNA (cytosine 5) methyltransferase 3A antibody

• DNA (cytosine-5)-methyltransferase 3A antibody

• 

  Western blot - Anti-Dnmt3a antibody (ab2850)Image from Nucleic Acids Res. 2014 Jan; 42(2): 804–821. Fig 5D, doi: 10.1093/nar/gkt920 Reproduced under the creative commons licence. https://creativecommons.org/licenses/by/4.0/

  Western blot analysis of DNMT3a in HeLa silenced cells. Total cell extracts were prepared 48 h after electroporation and analyzed by immunoblot with ab2850.

• 

  Western blot - Anti-Dnmt3a antibody (ab2850)

  Western blot of Dnmt3a on P19 cell nuclear extract using ab2850.

• 

  Western blot - Anti-Dnmt3a antibody (ab2850)

  All lanes : Anti-Dnmt3a antibody (ab2850) at 1/500 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : HEK293 cell lysate
  Lane 4 : A549 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ HRP at 1/1000 dilution
  
  Predicted band size: 101.5 kDa
  Observed band size: 120 kDawhy is the actual band size different from the predicted?

•