Anti-PSD93抗体
参阅全部 PSD93 一抗
兔多克隆抗体to PSD93
Rabbit
Detects Post Synaptic Density 93 (PSD 93) from rat tissues. This antibody does not detect other synapse-associated protein family members.
适用于: IHC-Fr, WB, ICC/IFmore details
与反应: Mouse, Rat, Human
Synthetic peptide corresponding to Rat PSD93 aa 350-450.
IHC-Fr: Rat cerebellum. WB: SH-SY5Y and Neuro-2a cell lysate.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
Post Synaptic Density 93 (PSD 93), also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD 93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD 93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. PSD 93 is believed to participate in the clustering of certain proteins, including N-methyl-D-aspartate (NMDA) receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD 93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with PSD 93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD 93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
多克隆
IgG
Abpromise™承诺保证使用ab2930于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 3 µg/ml. Detects a band of approximately 110 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Entrez Gene: 1740 Human
Entrez Gene: 23859 Mouse
Omim: 603583 Human
SwissProt: Q15700 Human
SwissProt: Q91XM9 Mouse
Unigene: 367656 Human
Unigene: 147213 Mouse
Unigene: 257035 Mouse
Unigene: 202966 Rat
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Immunohistochemistry (Frozen sections) - Anti-PSD93 antibody (ab2930)
Immunolocalization of PSD-93 in rat cerebellum using ab2930.
Western blot - Anti-PSD93 antibody (ab2930)
All lanes : Anti-PSD93 antibody (ab2930) at 3 µg/ml
Lane 1 : SH-SY5Y (human neuroblastoma cell line) whole cell lysate
Lane 2 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at 1/4000 dilution
Immunocytochemistry/ Immunofluorescence - Anti-PSD93 antibody (ab2930)
ICC/IF image of ab2930 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2930, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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