FITC Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody

• 产品名称

  FITC荧光Anti-DDDDK tag (Binds to FLAG® tag sequence)抗体
  参阅全部 DDDDK tag (Binds to FLAG® tag sequence) 一抗

• 描述

  FITC荧光兔多克隆抗体to DDDDK tag (Binds to FLAG® tag sequence)

• 宿主

  Rabbit

• 偶联物

  FITC. Ex: 493nm, Em: 528nm

• 经测试应用

  适用于: WBmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Synthetic peptide corresponding to DDDDK tag (Binds to FLAG® tag sequence). Peptide xxxDDDDK conjugated to KLH. xxx represents random amino acids. This sequence represents the Enterokinase Cleavage Site.

• 常规说明

  Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.

  The molar F/P ratio is 4.1.

  
  

  FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C.

• 存储溶液

  Preservative: 0.1% Sodium azide
  Constituent: 0.2% BSA

• 纯度

  Immunogen affinity purified

• 克隆

  多克隆

• 同种型

  IgG

The Abpromise guarantee

Abpromise™承诺保证使用ab2492于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用说明

Prior to conjugation the antibody was analyzed by western blot using various amino-terminal and carboxy-terminal DDDDK fusion proteins and Invitrogen Positope™ R900-40. A 1/5000 dilution gave bands against 0.1, 1.0 and 10 ng of the fusion proteins and 100 ng and 25 ng of the Positope.
Optimal working dilutions should be determined for your particular assay conditions.

别名

• DDDDK epitope tag antibody

• DDDK antibody

• ddk antibody

• DYKDDDDK (also known as the FLAG® tag sequence) antibody

• DYKDDDDK antibody

• DYKDDDDK epitope tag antibody

• DYKDDDDK tag antibody

• ECS epitope tag antibody

• ECS tag antibody

• Enterokinase Cleavage Site epitope tag antibody

• Enterokinase Cleavage Site tag antibody

• FLAG® antibody

• FLAG® tag antibody