Anti-c-Kit (phospho Y823)抗体
参阅全部 c-Kit 一抗
兔多克隆抗体to c-Kit (phospho Y823)
Rabbit
适用于: ICC, WBmore details
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Dog
Synthetic peptide corresponding to c-Kit (phospho Y823).
WB: M07e cells +/- SCF. ICC: Jurkat cells.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c Kit protein. The final product is generated by affinity chromatography using a c Kit derived peptide that is phosphorylated at tyrosine 823.
多克隆
IgG
Abpromise™承诺保证使用ab5634于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/250. | |
| WB | 1/1000. Detects a band of approximately 145 kDa. |
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SwissProt: P10721 Human
SwissProt: P05532 Mouse
Unigene: 479754 Human
Unigene: 247073 Mouse
Entrez Gene: 3815 Human
Entrez Gene: 16590 Mouse
Omim: 164920 Human
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Western blot - Anti-c-Kit (phospho Y823) antibody (ab5634)
Peptide Competition: Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5634 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5634 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from M07e cells left untreated (1) or treated (2-5) with SCF were resolved
Immunocytochemistry - Anti-c-Kit (phospho Y823) antibody (ab5634)
Jurkat cells stained for c-Kit cleave site (green) using ab5634 at 1/250 dilution in ICC/IF. It was followed by Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin at 1/300 dilution. Panel d represents the merged image showing membranous localization. Panel e shows the untreated control cells with no signal. Panel f shows the no primary antibody control.
