Anti-EGFR (phospho Y992)抗体
参阅全部 EGFR 一抗
兔多克隆抗体to EGFR (phospho Y992)
Rabbit
适用于: WB, IHC-P, ICCmore details
与反应: Human
Synthetic peptide corresponding to Human EGFR (phospho Y992).
A431 cells stimulated with EGF; human lung adenocarcinoma, human hepatocellular carcinoma
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free.
浓度
50 µl 浓度为 0.5 mg/ml
Immunogen affinity purified
The antibody has been negatively preadsorbed using (i) a non phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated epidermal growth factor receptor (EGFR), and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using an EGFR-derived peptide that is phosphorylated at tyrosine 922.
多克隆
IgG
Abpromise™承诺保证使用ab5638于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 170 kDa. | |
| IHC-P | 1/10 - 1/1000. | |
| ICC | 1/100 - 1/500. |
Entrez Gene: 1956 Human
Omim: 131550 Human
SwissProt: P00533 Human
Unigene: 488293 Human
Unigene: 605083 Human
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Western blot - Anti-EGFR (phospho Y992) antibody (ab5638)
All lanes : Anti-EGFR (phospho Y992) antibody (ab5638) at 1/1000 dilution
Lane 1 : A431 cells unstimulated
Lanes 2-5 : A431 cells stimulated with 200 ng/mL EGF for 15 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y992) antibody (ab5638)
Immunohistochemistry analysis of EGFR (pY992) with ab5638 at 1/20 dilution showing staining in the cytoplasm and membrane of paraffin-embedded human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).
Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y992) antibody (ab5638)
Immunohistochemistry analysis of EGFR (pY992) with ab5638 at 1/20 dilution showing staining in the cytoplasm and membrane of paraffin-embedded human hepatocellular carcinoma (right) compared to a negative control without primary antibody (left).
Antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Immunocytochemistry - Anti-EGFR (phospho Y992) antibody (ab5638)
Immunocytochemical analysis of EGFR [pY992] was performed using A-431 cells treated with 200 ng/mL of EGF for 10 minutes. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton™ X-100, and blocked with 1% BSA. The cells were labeled with ab5638 at 1/250 dilution and incubated overnight at 4°C followed by a Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) DAPI (Panel c: red) F-actin was stained with Rhodamine Phalloidin at 1/300. Panel d represents the merged image showing membranous localization. Panel e represents cells treated with antagonist, Afatinib (1µM for 6hrs) followed by EGF (200 ng/mL for 10 minutes), showing reduced Phospho-EGFR staining. Panel f shows untreated cells with no signal. Panel g represents control cells with no primary antibody to assess background.
