Anti-PERP抗体
参阅全部 PERP 一抗
兔多克隆抗体to PERP
Rabbit
适用于: ICC/IF, WBmore details
与反应: Human
预测可用于: Mouse
Synthetic peptide:
EDDLLGNAKPRYFYTSA
, corresponding to amino acids 175-193 of Human PERP.
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
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Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS
浓度
100 µg 浓度为 1 mg/ml
Immunogen affinity purified
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in the p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2, MTBP and PERP, were identified and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein. p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis. The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family.
多克隆
IgG
Abpromise™承诺保证使用ab5986于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) | Use a concentration of 10 µg/ml. |
| WB | Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 21 kDa. |
Entrez Gene: 64065 Human
Entrez Gene: 64058 Mouse
Omim: 609301 Human
SwissProt: Q96FX8 Human
SwissProt: Q9JK95 Mouse
Unigene: 201446 Human
Unigene: 28209 Mouse
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KCP1 antibody
Keratinocyte associated protein 1 antibody
Keratinocyte-associated protein 1 antibody
Keratinocytes associated protein 1 antibody
KRTCAP 1 antibody
KRTCAP1 antibody
p53 apoptosis effector related to PMP 22 antibody
p53 apoptosis effector related to PMP-22 antibody
p53 apoptosis effector related to PMP22 antibody
P53 induced protein PIGPC1 antibody
P53-induced protein PIGPC1 antibody
Perp antibody
PERP TP53 apoptosis effector antibody
PERP_HUMAN antibody
PIGPC 1 antibody
PIGPC1 antibody
RP3 496H19.1 antibody
THW antibody
TP53 apoptosis effector antibody
Transmembrane protein THW antibody
1110017A08Rik antibody
dJ496H19.1 antibody
KCP 1 antibody
Western blot - Anti-PERP antibody (ab5986)
All lanes : Anti-PERP antibody (ab5986) at 1 µg
Lane 1 : 293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : K569 cell lysate
Lane 6 : MCF-7 cell lysate
Lane 7 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Western blot - Anti-PERP antibody (ab5986)
Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml. Western blot analysis of PERP expression in A431 whole cell lysates in the absence (A) and presence (B) of blocking peptide with anti- PERP at 1 µg /ml.
Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody (ab5986)
Immunocytochemistry of PERP in A431 cells with PERP antibody at 10 µg/ml.
