Anti-PDGFR alpha + Beta (phospho Y572 + Y574)抗体
兔多克隆抗体to PDGFR alpha + Beta (phospho Y572 + Y574)
Rabbit
适用于: ICC, WBmore details
与反应: Mouse, Human
预测可用于: Rat, Xenopus laevis
Synthetic peptide corresponding to Human PDGFR alpha + Beta aa 550-650 (phospho Y572 + Y574). This corresponds to Tyrosine 579 and 581 of Human PDGFR beta. The sequence is conserved in PDGFR alpha mouse, rat and frog.
WB: NIH/3T3 cells +/- PDGF; ICC: MCF7 cells treated with PDGF .
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA
BSA is IgG and protease free
浓度
50 µl 浓度为 0.25 mg/ml
Immunogen affinity purified
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha / beta. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at Tyrosines 572 and 574.
多克隆
IgG
Abpromise™承诺保证使用ab5443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | 1/100 - 1/500. | |
| WB | 1/1000. Detects a band of approximately 170 - 185 kDa. |
Entrez Gene: 5156 Human
Entrez Gene: 5159 Human
Entrez Gene: 18595 Mouse
Entrez Gene: 18596 Mouse
Omim: 173410 Human
Omim: 173490 Human
SwissProt: P09619 Human
SwissProt: P16234 Human
SwissProt: P05622 Mouse
SwissProt: P26618 Mouse
CD140A antibody
CD140a antigen antibody
CD140b antibody
CD140b antigen antibody
PDGF Receptor alpha + beta antibody
PDGFR 2 antibody
PDGFR alpha antibody
PDGFR beta antibody
PDGFRA antibody
PDGFRB antibody
Platelet derived growth factor receptor alpha antibody
Platelet derived growth factor receptor beta antibody
Western blot - Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody (ab5443)
Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5443 antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5443 blocks the antibody signal, thereby demonstrating the specificity of the antibody. Peptide Competition: Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF
Immunocytochemistry - Anti-PDGFR alpha + Beta (phospho Y572 + Y574) antibody (ab5443)
Immunocytochemistry analysis of 70% confluent log phase MCF7 cells treated with PDGF (50 ng/mL for 10 min) labeling PDGFR alpha + Beta (phospho Y572 + Y574) with ab5443. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab5443 at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor® 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1/400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 594 Phalloidin. Panel d is a merged image showing cytoplasmic and membrane localization. Panel e shows untreated cells. Panel f shows no primary antibody control. The images were captured at 20X magnification.
