Anti-6X His tag® antibody [4D11]

• 产品名称

  Anti-6X His tag®抗体[4D11]
  参阅全部 6X His tag® 一抗

• 描述

  小鼠单克隆抗体[4D11] to 6X His tag®

• 宿主

  Mouse

• 经测试应用

  适用于: Dot blot, ELISA, WB, IP, ICC/IF, Flow Cytmore details

• 种属反应性

  与反应: Species independent
  

• 免疫原

  Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

• 表位

  This antibody was shown to recognize an epitope (HHHHHHGS) encoded by the polylinker of several expression vectors (including pDS:RBSII, pQE-30, and pQE-50).

• 常规说明

  
  

  This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

  
  

  HIS-TAG® is a trademark of EMD Biosciences, Inc.

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.40
  Preservative: 0.02% Sodium azide
  Constituents: 6.97% L-Arginine, PBS

• 浓度

• 100 µg 浓度为 1 mg/ml

• 纯度

  Affinity purified

• 克隆

  单克隆

• 克隆编号

  4D11

• 骨髓瘤

  NS1

• 同种型

  IgG2a

• 轻链类型

  kappa

The Abpromise guarantee

Abpromise™承诺保证使用ab5000于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

别名

• 6 His epitope tag antibody

• Hexa His tag antibody

• HHHHHH epitope tag antibody

• HHHHHH tag antibody

• His tag antibody

• Polyhistidine Tag antibody

• 

  Western blot - Anti-6X His tag® antibody [4D11] (ab5000)

  Anti-6X His tag® antibody [4D11] (ab5000) at 1/1000 dilution + 6X His Tagged Protein at 1 µg
  
  Secondary
  Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/3000 dilution
  
  Developed using the ECL technique.
  
  Performed under reducing conditions.
  
  Exposure time: 30 seconds
  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [4D11] (ab5000)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

  ab5000 staining Human HEK 293 cells transfected with CACNB3-His by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 5% serum for 20 minutes at 25°C. The primary antibody (1/250 in 1% goat serum, 0.1% TX100, 1X PBS) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 546 conjugated goat anti-mouse antibody (1/500) was used as the secondary.  DAPI was used for nuclei staining (blue).

  See Abreview

• 

  Western blot - Anti-6X His tag® antibody [4D11] (ab5000)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

  All lanes : Anti-6X His tag® antibody [4D11] (ab5000) at 1/2000 dilution
  
  Lane 1 : Lysates prepared from HEK-293 cells
  Lane 2 : Lysates prepared from HEK-293 cells transfected with Beta3-His (CACNB3)
  
  Secondary
  All lanes : HRP-conjugated goat anit-mouse IgG polyclonal at 1/5000 dilution
  

  See Abreview

• 

  Immunoprecipitation - Anti-6X His tag® antibody [4D11] (ab5000)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic

  ab5000 Immunoprecipitate in human HEK293 whole cell lysate. 25µg of cell lysate incubated with primary antibody (undiluted) and matrix (Protein G) for 16 hours at 4°C. For western blotting an undiluted HRP-Goat anti-rabbit IgG polyclonal was used. Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti beta3 Ab

  Lane 1. Lysate of HEK293 cells expressing beta3-His (CACNB3)

  Lane 2. IP with anti His Ab

  Lane 3. Non bound fraction

  See Abreview