Anti-Fibronectin抗体
参阅全部 Fibronectin 一抗
兔多克隆抗体to Fibronectin
Rabbit
适用于: ICC/IF, WB, IHC-Pmore details
与反应: Mouse, Human
Full length native protein (purified) corresponding to Human Fibronectin. Fibronectin isolated from a pool of normal human plasma.
WB: Human colon tissue lysate, HepG2, NIH 3T3 whole cell lysate. IHC-P: Human kidney tissue. ICC: HeLa cells.
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Liquid
Shipped at 4°C. Store at +4°C.
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 99.854% PBS, 0.096% BSA
Affinity purified
多克隆
IgG
unknown
Abpromise™承诺保证使用ab2413于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (28) | Use at an assay dependent concentration. |
| WB | (31) | Use at an assay dependent concentration. Detects a band of approximately 285 kDa (predicted molecular weight: 262 kDa). |
| IHC-P | (14) | Use at an assay dependent concentration. |
Entrez Gene: 2335 Human
Entrez Gene: 14268 Mouse
Omim: 135600 Human
SwissProt: P02751 Human
SwissProt: P11276 Mouse
Unigene: 203717 Human
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Fibronectin with ab2413 at 1/50 dilution. Antigen retrieval performed with EDTA buffer pH 8 before commencing with IHC staining protocol.
Western blot - Anti-Fibronectin antibody (ab2413)
All lanes : Anti-Fibronectin antibody (ab2413) at 1 µg/ml
Lane 1 : Human colon tissue lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 262 kDa
Observed band size: 285 kDawhy is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue, staining Fibronectin with ab2413.
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody (ab2413)
ICC/IF image of ab2413 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2413, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody (ab2413)
ab2413 staining Fibronectin in human kidney tissue section by IHC-P (Formalin/PFA-fixed paraffin embedded tissue sections). Tissue sections were incubated with ab2413 at a dilution of 1:250 for one hour. Heat mediated antigen retrieval technique was used with citrate buffer at pH 6.0. DAB staining was done with a biotinylated secondary for 45 min at RT at a concentration of 1:1000.
