Anti-ERCC1 antibody [8F1]

• 产品名称

  Anti-ERCC1抗体[8F1]
  参阅全部 ERCC1 一抗

• 描述

  小鼠单克隆抗体[8F1] to ERCC1

• 宿主

  Mouse

• 经测试应用

  适用于: ICC/IF, IHC-P, Flow Cytmore details

• 种属反应性

  与反应: Human
  

• 免疫原

  Recombinant full length protein corresponding to Human ERCC1.

• 阳性对照

• Tonsil.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  pH: 7.3
  Preservative: 0.05% Sodium azide
  Constituent: 1% BSA

• 纯度

  IgG fraction

• 克隆

  单克隆

• 克隆编号

  8F1

• 骨髓瘤

  unknown

• 同种型

  IgG2b

• 轻链类型

  unknown

The Abpromise guarantee

Abpromise™承诺保证使用ab2356于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 数据库链接

• Entrez Gene: 2067 Human

• Omim: 126380 Human

• SwissProt: P07992 Human

• Unigene: 435981 Human

• 别名

• DNA excision repair protein ERCC-1 antibody

• DNA excision repair protein ERCC1 antibody

• ERCC 1 antibody

• ERCC1 antibody

• ERCC1_HUMAN antibody

• Excision repair cross complementation group 1 antibody

• Excision repair cross complementing 1 antibody

• Excision Repair Cross Complementing Rodent Repair Deficiency Complementation Group 1 antibody

• Excision repair protein antibody

• RAD 10 antibody

• RAD10 antibody

• UV 20 antibody

• UV20 antibody

• COFS 4 antibody

• COFS4 antibody

• DNA excision repair protein ERCC 1 antibody

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (ab2356)

  Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue with ab2356 labelling ERCC1.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ERCC1 antibody [8F1] (ab2356)

  ICC/IF image of ab2356 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2356, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  

• 

  Flow Cytometry - Anti-ERCC1 antibody [8F1] (ab2356)

  Overlay histogram showing HeLa cells stained with ab2356 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2356, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
  
  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERCC1 antibody [8F1] (ab2356)

  Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue sections with ab2356 labelling ERCC1.