Anti-Peripherin antibody [8G2]

• 产品名称

  Anti-Peripherin抗体[8G2]
  参阅全部 Peripherin 一抗

• 描述

  小鼠单克隆抗体[8G2] to Peripherin

• 宿主

  Mouse

• 经测试应用

  适用于: WB, ICC/IFmore details

• 种属反应性

  与反应: Mouse, Rat, Cow, Human
  

• 免疫原

  Recombinant full length protein corresponding to Rat Peripherin.
  Database link: P21807

• 阳性对照

• WB: Mouse, rat, cow spinal cord lysate, SH-SY5Y, PC-12 whole cell lysate. ICC: SH-SY5Y, PC-12 cells.

• 常规说明

  
  

  The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

  If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

• 形式

  Liquid

• 存放说明

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

• 存储溶液

  Preservative: 0.032% Sodium azide
  Constituents: 49.98% Glycerol, 49.98% PBS

• 纯度

  Protein A purified

• 纯化说明

  Purified antibody from TCS.

• 克隆

  单克隆

• 克隆编号

  8G2

• 同种型

  IgG1

• 轻链类型

  kappa

• The Abpromise guarantee

• Abpromise™承诺保证使用ab4653于以下的经测试应用

• “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

• 应用	Ab评论	说明
  WB		1/5000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
  ICC/IF		1/500.

• 数据库链接

• Entrez Gene: 510082 Cow

• Entrez Gene: 5630 Human

• Entrez Gene: 19132 Mouse

• Entrez Gene: 24688 Rat

• Omim: 170710 Human

• SwissProt: P41219 Human

• SwissProt: P15331 Mouse

• SwissProt: P21807 Rat

• Unigene: 37044 Human

• Unigene: 2477 Mouse

• Unigene: 11086 Rat

别名

• NEF 4 antibody

• NEF4 antibody

• Neurofilament 4 (57kD) antibody

• Neurofilament 4 antibody

• Perf antibody

• PERI_HUMAN antibody

• Peripherin antibody

• PRPH 1 antibody

• prph antibody

• PRPH1 antibody




• 

  Western blot - Anti-Peripherin antibody [8G2] (ab4653)

  All lanes : Anti-Peripherin antibody [8G2] (ab4653) at 1/500 dilution
  
  Lane 2 : Mouse spinal cord lysate
  Lane 3 : Rat spinal cord lysate
  Lane 4 : Cow spinal cord lysate
  Lane 5 : SH-SY5Y (human bone marrow neuroblastoma cell) whole cell lysate
  Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
  
  Secondary
  All lanes : Anti-Mouse IgG
  
  Predicted band size: 57 kDa
  Observed band size: 57 kDa
  Additional bands at: 25 kDa (possible IgG), 50 kDa (possible IgG)
  
  
  
  

  The band at ~57kDa corresponds to the peripherin protein. Bands at 50 and 25kDa detected in the mouse spinal cord lysate, correspond to the heavy and light chains of mouse IgG.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Peripherin antibody [8G2] (ab4653)

  Immunofluorescent analysis of SH-SY5Y (human bone marrow neuroblastoma cell) cells (A) and PC-12 (rat adrenal gland pheochromocytoma cell) cells (B), stained with ab4653 at 1/500 dilution (Red) and costained with chicken polyclonal anti-vimentin antibody at 1/10,000 dilution (Green). Blue: DAPI staining of nuclear DNA.