Anti-Tau (phospho S199)抗体
参阅全部 Tau 一抗
兔多克隆抗体to Tau (phospho S199)
Rabbit
The specificity of this antibody refers to P10636-8.
适用于: IHC-P, WBmore details
与反应: Mouse, Human, African green monkey
预测可用于: Rat
Synthetic peptide corresponding to Human Tau (phospho S199).
Database link: P10636-8
IHC-P: Mouse brain tissue, human brain tissue. WB: African green monkey kidney.
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Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
浓度
50 µl 浓度为 0.1 mg/ml
Immunogen affinity purified
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated tau. The final product is generated by affinity chromatography using a tau-derived peptide that is phosphorylated at serine 199.
多克隆
IgG
Abpromise™承诺保证使用ab4749于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/20 - 1/200. | |
| WB | (1) | 1/1000. Detects a band of approximately 60 kDa. |
Entrez Gene: 4137 Human
Entrez Gene: 17762 Mouse
Omim: 157140 Human
SwissProt: P10636 Human
SwissProt: P10637 Mouse
Unigene: 101174 Human
Unigene: 1287 Mouse
Unigene: 2455 Rat
There are 9 isoforms produced by alternative splicing.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S199) antibody (ab4749)
Paraffin embedded human brain tissue (right) stained for Tau using ab4749 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tau (phospho S199) antibody (ab4749)
Paraffin embedded mouse brain tissue (right) stained for Tau using ab4749 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
Western blot - Anti-Tau (phospho S199) antibody (ab4749)
Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c). Cell extracts from African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and a protein phosphatase inhibitor, were resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins were transferred to nitrocellulose. Membranes were incubated with 0.50 µg/mL anti-phospho tau [pS199] (ab4749), following prior incubation in the absence (a) or presence of the peptide immunogen (b), or the nonphosphopeptide corresponding to the tau phosphopeptide (c).
